Repository of Research and Investigative Information

Repository of Research and Investigative Information

Zahedan University of Medical Sciences

Apoptosis and Reduced Cell Proliferation of HL-60 Cell Line Caused by Human Telomerase Reverse Transcriptase Inhibition by siRNA

(2010) Apoptosis and Reduced Cell Proliferation of HL-60 Cell Line Caused by Human Telomerase Reverse Transcriptase Inhibition by siRNA. Acta Haematologica. pp. 72-78. ISSN 0001-5792

Full text not available from this repository.

Official URL: <Go to ISI>://WOS:000282059300002

Abstract

Background: The close correlation between telomerase activity and human telomerase reverse transcriptase (hTERT) expression has made hTERT to be considered as a selective molecular target for human cancer therapy. In this study, the ability of short-interfering RNA (siRNA) to downregulate hTERT expression and its correlation with cell growth and apoptosis in the promyelocytic cell line HL-60 was evaluated. Materials and Methods: hTERT siRNA was designed and transfected to HL-60. hTERT mRNA expression, cell proliferation and apoptotic cells were measured. Results and Conclusion: The results indicated that hTERT siRNA resulted in 97.2 +/- 8 0.6 downregulation of the hTERT mRNA content; inhibition of the cell proliferation rate was about 52.8 +/- 2.3 and the apoptotic index of cells was 30.5 +/- 1.5. hTERT plays an essential role in cell proliferation and control of the viability of leukemic cells, thus promising the development of drugs for leukemia. Copyright (c) 2010 S. Karger AG, Basel

Item Type: Article
Keywords: Apoptosis Cell proliferation HL-60 Human telomerase reverse transcriptase Short-interfering RNA Telomerase down-regulation leukemia-cells cancer rna expression htert growth gene loop Hematology
Page Range: pp. 72-78
Journal or Publication Title: Acta Haematologica
Journal Index: ISI
Volume: 124
Number: 2
Identification Number: https://doi.org/10.1159/000314960
ISSN: 0001-5792
Depositing User: خانم مهدیه رضائی پور
URI: http://eprints.zaums.ac.ir/id/eprint/2780

Actions (login required)

View Item View Item