(2010) Effect of MDR1 polymorphism on multidrug resistance expression in breast cancer patients. Genetics and Molecular Research. pp. 34-40. ISSN 1676-5680
Full text not available from this repository.
Abstract
One of the limitations in the treatment of cancer patients with chemotherapy is the development of multidrug resistance (MDR). A well-known mechanism responsible for drug resistance is over-expression of ABC-transporter genes such as MDR1. This gene encodes p-glycoprotein (P-gp), a transmembrane glycoprotein that transports many hydrophobic substrates and anti-cancer drugs out of the cell. MDR1 gene polymorphisms could alter the expression level of P-gp and consequently result in drug resistance. We investigated a possible association between MDR1 gene C3435T polymorphism and its expression in Iranian breast cancer patients. PCR-RFLP was used for the detection of C3435T single nucleotide polymorphism in 54 breast cancer patients and 50 healthy individuals. The expression level of MDR1 was determined by real-time quantitative PCR. We observed no difference in the frequency of C3435T polymorphism between breast cancer patients and healthy controls. However, there was a significant association between MDR1 expression levels and C3435T polymorphism in the patients. C3435T polymorphism may play a role in inducing drug resistance by altering the expression level of the MDR1 gene.
Item Type: | Article |
---|---|
Keywords: | Multidrug resistance MDR1 gene C3435T polymorphism Real-time polymerase chain reaction Breast cancer p-glycoprotein expression c3435t polymorphism gene transporters leukemia Biochemistry & Molecular Biology Genetics & Heredity |
Page Range: | pp. 34-40 |
Journal or Publication Title: | Genetics and Molecular Research |
Journal Index: | ISI |
Volume: | 9 |
Number: | 1 |
Identification Number: | https://doi.org/10.4238/vol9-1gmr669 |
ISSN: | 1676-5680 |
Depositing User: | خانم مهدیه رضائی پور |
URI: | http://eprints.zaums.ac.ir/id/eprint/2783 |
Actions (login required)
![]() |
View Item |