Repository of Research and Investigative Information

Repository of Research and Investigative Information

Zahedan University of Medical Sciences

Association of pfcrt but not pfmdr1 Alleles with chloroquine resistance in Iranian isolates of Plasmodium falciparum

(2008) Association of pfcrt but not pfmdr1 Alleles with chloroquine resistance in Iranian isolates of Plasmodium falciparum. American Journal of Tropical Medicine and Hygiene. pp. 633-640. ISSN 0002-9637

Full text not available from this repository.

Official URL: <Go to ISI>://WOS:000254943300021

Abstract

This study was designed to analyze the Plasmodium falciparum chloroquine resistance transporter (pfcrt) and P. falciparum multidrug resistance 1 (ptindr1) mutations as markers of chloroquine (CO) resistance in 200 blood samples collected from malaria patients in south-eastern Iran during 2002-2005. Among these, 25 (post-treatment) fulfilled the 28-day follow-up study. A high number of Iranian P. falciparum (97) strains harbored quadruple inutations at codons 76T, 220S, 326D, and 356L. All post-treatment isolates harbored the mutant allele 76T, but low rates of the mutant allele 86Y (44) of the pfmdr1 gene were detected. No wild haplotype of pfcrt (72-CVMNKAQNIR-371) was found in post-treatment samples; however, 56 of clinical "failure" samples carried the wild type of pfmdr1 (NYSND). The present results suggest a strong association between pfcrt 76T, but not pfmdr1 86Y mutation and in vivo CQ resistance. Furthermore, we found the CQ resistance-associated SVMNT haplotype, which previously had been seen in South American isolates. Although Iran is located more proximally to Southeast Asia than to South America, no CQ resistance-associated MET haplotye has been observed in this region. Therefore, these results were not consistent with the earlier presumed spread of CQR parasites from Southeast Asia to Africa via the Indian subcontinent. In conclusion, P. falciparum mutations associated with resistance to CO are abundant in south-eastern Iran and this finding strongly supports that CQ as the first line drug is inadequate for treatment of uncomplicated falciparum malaria in Iran.

Item Type: Article
Keywords: gene haplotype svmnt multidrug-resistance molecular epidemiology malaria parasites transporter gene drug-resistance point mutations high prevalence southeast-asia in-vivo Public, Environmental & Occupational Health Tropical Medicine
Page Range: pp. 633-640
Journal or Publication Title: American Journal of Tropical Medicine and Hygiene
Journal Index: ISI
Volume: 78
Number: 4
Identification Number: https://doi.org/10.4269/ajtmh.2008.78.633
ISSN: 0002-9637
Depositing User: خانم مهدیه رضائی پور
URI: http://eprints.zaums.ac.ir/id/eprint/2858

Actions (login required)

View Item View Item