Repository of Research and Investigative Information

Repository of Research and Investigative Information

Zahedan University of Medical Sciences

Isolation and molecular characterization of Toxoplasma gondii strains from different hosts in Iran

(2007) Isolation and molecular characterization of Toxoplasma gondii strains from different hosts in Iran. Parasitology Research. pp. 111-115. ISSN 0932-0113

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Official URL: <Go to ISI>://WOS:000246099600015

Abstract

Toxoplasma gondii is one of the most prevalent protozoan parasites in Iran. This study was aimed to isolate T. gondii from a variety of hosts and to genetically analyze the parasite isolates. The prevalence of T. gondii in different animal hosts was assessed in two provinces of Iran, Tehran and Mazandaran in the central and northern parts, respectively. The latex agglutination (LA) test was carried out, and antibodies were found in 24 out of 105 sheep, 5 out of 35 goats, 23 out of 45 free-ranging chickens (Gallus domesticus), 2 out of 13 ducks (Anas spp.), and two of four stray cats (Felis domesticus). T. gondii was isolated by bioassay in mice from four sheep, six chickens, one duck, two cats, and three human samples. Genotyping of these 16 isolates was performed using Multiplex PCR for five microsatellite markers and GRA6 gene sequence analysis. The results indicated that the studied isolates consisted of only two genotypes, II and III, with no evidence of type 1 or mixed genotypes.

Item Type: Article
Keywords: chickens sheep seroprevalence cattle pcr Parasitology
Page Range: pp. 111-115
Journal or Publication Title: Parasitology Research
Journal Index: ISI
Volume: 101
Number: 1
Identification Number: https://doi.org/10.1007/s00436-007-0461-7
ISSN: 0932-0113
Depositing User: خانم مهدیه رضائی پور
URI: http://eprints.zaums.ac.ir/id/eprint/2889

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