Repository of Research and Investigative Information

Repository of Research and Investigative Information

Zahedan University of Medical Sciences

The effect of hyaluronic acid on motility, vitality and fertilization capability of mouse sperms after cryopreservation

(2007) The effect of hyaluronic acid on motility, vitality and fertilization capability of mouse sperms after cryopreservation. Iranian Journal of Reproductive Medicine. pp. 45-50. ISSN 1680-6433

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Official URL: <Go to ISI>://WOS:000254385400002

Abstract

Background: Various approaches have been used in the attempts to improve the quality of frozen-thawed mouse sperms. According to literatures, it seems that hyaluronic acid (HA) has an important role on the permeability and motility of sperms and their interaction with gametes. Objective: For evaluation of HA supplementation on sperm characteristics and fertilization capability, we investigated the effect of different doses of HA on mouse sperm morphology, motility, vitality and fertilization capability after freezing and thawing. Materials and Methods: The cauda epididymes was removed from 6 male mice with aseptic method. The sperm samples were frozen in 1.8 ml cryotubes with 18 raffinose and 3 skimmed milk containing cryo-protectant solution. HA at the concentration of 750, 1000 or 1250 mu g/ml was supplemented to frozen-thawed sperms. Sperm motility was measured with microscope, and fertilization rate was evaluated after routine IVF by counting the fertilized oocytes. For sperm morphology, papaniclau staining was used while; Eosin B was used for the assessment of sperm viability rate. Results: HA supplementation (750 mu g/ml) improved motility parameters (P < 0.05) and increased the fertility rate (p < 0.05). The effect of 1,000 mu g/ml HA was also positive on the sperms. But 1,250 mu g/ml HA had negative effect on above mentioned characteristic. On the other hand, none of these doses had any effect on sperm morphology. Conclusion: The dose of 750 mu g/ml of HA has the greatest effect on the motility, vitality and fertility rate of sperms after cryopreservation.

Item Type: Article
Keywords: hyaluronic acid (HA) cryopreservation in vitro fertilization (IVF) sperm parameters binding protein-1 habp1 boar spermatozoa in-vitro cellular maturity ability mice glycosaminoglycans capacitation penetration mutagenesis Obstetrics & Gynecology
Page Range: pp. 45-50
Journal or Publication Title: Iranian Journal of Reproductive Medicine
Journal Index: ISI
Volume: 5
Number: 2
ISSN: 1680-6433
Depositing User: خانم مهدیه رضائی پور
URI: http://eprints.zaums.ac.ir/id/eprint/2894

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