Repository of Research and Investigative Information

Repository of Research and Investigative Information

Zahedan University of Medical Sciences

High rate of detection of mixed infections of Plasmodium vivax and Plasmodium falciparum in South-East of Iran, using nested PCR

(2007) High rate of detection of mixed infections of Plasmodium vivax and Plasmodium falciparum in South-East of Iran, using nested PCR. Parasitology International. pp. 61-64. ISSN 1383-5769

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Official URL: <Go to ISI>://WOS:000244856200009

Abstract

Sistan and Baluchestan province, South-East of Iran, has been reported as an endemic area of malaria Sadrizadeh B. Malaria in the world, in the eastern Mediterranean region and in Iran: Review article. WHO/EMRO Report 2001: 1-13.. The main objective of this research was to perform rapid and correct diagnoses of malaria infection. Blood specimens were collected from 140 suspected volunteers. The Giemsa-stained slides examination and nested PCR for amplification of the Plasmodium small subunit ribosomal genes (ssrRNA) were utilized. The results demonstrated 118 out of 140 cases (84.3%) positive for malaria parasites, including 60.7%, 20.7% and 2.9% as having Plasmodium vivax (P.v), Plasmodium falciparum (P.f) and mixed infections (P.v+P.f), respectively by microscopy. The nested PCR detected malaria parasites in 134 samples (94.3%), consisting of 51.4% P.v, 12.6% P.f and 29.3% mixed infections. The PCR analysis detected 37 cases of mixed infections more than that of the routine microscopy. These results suggested that there are a considerable number of cases with mixed infections in the study area that mainly remain undiagnosed by microscopy. It is also concluded that the nested PCR is a suitable complement to microscopy for accurate specific diagnosis of malaria species in field. (c) 2007 Published by Elsevier Ireland Ltd.

Item Type: Article
Keywords: Iran malaria nested PCR microscopy mixed infections polymerase-chain-reaction human malaria parasites papua-new-guinea sensitivity chloroquine resistance blood Parasitology
Page Range: pp. 61-64
Journal or Publication Title: Parasitology International
Journal Index: ISI
Volume: 56
Number: 1
Identification Number: https://doi.org/10.1016/j.parint.2006.12.001
ISSN: 1383-5769
Depositing User: خانم مهدیه رضائی پور
URI: http://eprints.zaums.ac.ir/id/eprint/2895

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