Repository of Research and Investigative Information

Repository of Research and Investigative Information

Zahedan University of Medical Sciences

Trypsin inhibitory capacity in vernal keratoconjunctivitis

(2007) Trypsin inhibitory capacity in vernal keratoconjunctivitis. Investigative Ophthalmology & Visual Science. pp. 264-269. ISSN 0146-0404

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Official URL: <Go to ISI>://WOS:000243355100035


PURPOSE. To study tear trypsin inhibitory capacity (T-TIC), serum trypsin inhibitory capacity (S-TIC) and their relationship to matrix metalloproteinases (MMP)-1 and -9 in patients with vernal keratoconjunctivitis (VKC). METHODS. In the first phase of the study, inactivation of alpha-1 antitrypsin (AAT) by MMP-1 and -9 was investigated in vitro. Subsequently, tear samples were collected after clinical evaluation from 14 patients with active VKC and 15 normal control subjects. Tear cytology was performed on all samples. Levels of T-TIC and S-TIC were determined by spectrophotometry, whereas levels of tear pro-MMP-1, pro-MMP-9, and active MMP-1 and -9 were determined by enzyme-linked immunosorbent assay (ELISA). RESULTS. MMP-1 and -9 inactivated AAT in vitro. S-TIC was significantly higher (P < 0.0001), and T-TIC (P < 0.0001) significantly lower in VKC samples. Tear levels of pro-MMP-1 and pro-MMP-9, and the activity of MMP-1 and -9 was significantly greater in patients with VKC than in healthy subjects (P < 0.0001). There was no significant correlation between T-TIC, MMP-1, and MMP-9 activity. In addition, T-TIC was not correlated to the total clinical score or to single clinical sign scores. CONCLUSIONS. In this study, tear trypsin inhibitory capacity was shown to be reduced and tear MMP-1 and -9 activity increased in patients with VKC. Although T-TIC did not correlate with VKC severity, a local reduced inhibitory capacity of ATT may facilitate or prolong conjunctival inflammation in VKC.

Item Type: Article
Keywords: plasma alpha-1-proteinase inhibitor mast-cell chymase alpha-1-antitrypsin deficiency allergic conjunctivitis proteinase-inhibitors molecular-basis serum-albumin angiotensin-i tear conversion Ophthalmology
Page Range: pp. 264-269
Journal or Publication Title: Investigative Ophthalmology & Visual Science
Journal Index: ISI
Volume: 48
Number: 1
Identification Number:
ISSN: 0146-0404
Depositing User: خانم مهدیه رضائی پور

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