Repository of Research and Investigative Information

Repository of Research and Investigative Information

Zahedan University of Medical Sciences

Genotyping of Hepatitis B Virus by Multiplex PCR in Sistan and Baluchestan Province

(2016) Genotyping of Hepatitis B Virus by Multiplex PCR in Sistan and Baluchestan Province. Zahedan Journal of Research in Medical Sciences. pp. 1-5. ISSN 2383-2894

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Abstract

Background: Hepatitis B virus (HBV) has been classified into 8 genotypes (A - H) according to at least 8% difference in complete nucleotide sequences or more than 4% in S gene. Different genotypes of HBV have important roles in development of certain disorders as: hepatitis, cirrhosis and hepatocellular carcinoma. Therefore, knowing the distribution of HBV genotypes, have considerable impacts on treatment strategies, vaccination program, diagnosis and prevention. Objectives: This study aims to determine the prevalence of HBV genotypes in patients infected with the virus in Sistan and Baluchestan province (south-east Iran). Patients and Methods: In this descriptive study, 163 patients infected with HBV were selected over the first 6 months (beginning fromMarch 2012 onward). All the patients were HBsAg (hepatitis B surface antigen) and HBeAg (extracellular form of HBcAg) positive along with viral load > 10000 copies/mL. After DNA extraction from the patients’ sera, viral genotypes were determined bymultiplex polymerase chain reaction (PCR) using special primers for each genotype. Results: From 163 samples, 154 samples (94.5%) had D genotype which was dominant genotype; and 9 samples (5.5%) had mixed genotype of C/D. Conclusions: This study showed that in Sistan and Baluchestan, like other regions of Iran as well as Mediterranean countries, D genotype is dominant genotype. In additional, C/D mixed genotype is also seen less frequently in this province.

Item Type: Article
Keywords: Genotype, Hepatitis B Virus, Multiplex, Polymerase Chain Reaction
Subjects: QW Microbiology and Immunology > QW 1-300 Microbiology
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Microbiology
Page Range: pp. 1-5
Journal or Publication Title: Zahedan Journal of Research in Medical Sciences
Journal Index: ISC
Volume: 18
Number: 2
Identification Number: https://doi.org/10.17795/zjrms-5986
ISSN: 2383-2894
Depositing User: خانم شهره شهرکی
URI: http://eprints.zaums.ac.ir/id/eprint/3152

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