Repository of Research and Investigative Information

Repository of Research and Investigative Information

Zahedan University of Medical Sciences

High prevalence of blaCMY AmpC Beta-Lactamase in ESBL Co-Producing Escherichia coli and Klebsiella spp. Clinical Isolates in Northeast of Iran

(2020) High prevalence of blaCMY AmpC Beta-Lactamase in ESBL Co-Producing Escherichia coli and Klebsiella spp. Clinical Isolates in Northeast of Iran. Journal of global antimicrobial resistance. ISSN 2213-7173 (Electronic) 2213-7165 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/32247080

Abstract

OBJECTIVES: Production of beta-lactamase enzymes such as AmpC beta-lactamases and Extended-Spectrum beta-Lactamases (ESBLs) is among the main mechanisms for resistance to expanded-spectrum cephalosporins. The present study was conducted to investigate prevalence and molecular epidemiology of plasmid mediated AmpC beta-lactamase in ESBL co-producing Escherichia coli (E.coli) and Klebsiella spp. (Klebsiella pneumoniae and Klebsiella oxytoca) clinical isolates in northeast of Iran. METHODS: A total of 602 E.coli and Klebsiella spp. clinical isolates were collected from three hospitals in Mashhad (northeast of Iran). Combination disk test was performed for phenotypic detection of ESBLs. Screening for detection of AmpC beta-lactamases was performed by susceptibility test to cefoxitin disc among ESBL producing isolates. Confirmatory test for AmpC beta-lactamases was performed by The Mast(R) D68C test. Identification of plasmid mediated AmpC cluster genes was done by multiplex PCR. RESULTS: Among 336 ESBL-producing strains, 230 (68.4) isolates were resistant to cefoxitin. Results of the Mast(R) D68C test showed that 30 (69/230) of cefoxitin resistant isolates simultaneously exhibited ESBL and AmpC activity and 22 (51/230) of isolates probably showed MDR phenotype. Results of multiplex PCR among ESBL-positive isolates showed that, 16.7 (56/336) of isolates were positive for plasmid-borne ampC cluster genes, and CMY (38) was the most frequent genotype of plasmid mediated AmpC. CONCLUSION: Findings of the study revealed that an increase in the prevalence of ESBL and AmpC co-producer in E. coli and Klebsiella spp strains may become an important public health issue. Therefore, there is a vital need for surveillance of spread of these clinical isolates.

Item Type: Article
Keywords: AmpC beta-Lactamases Escherichia coli Extended Spectrum beta-Lactamases (ESBLs) Klebsiella spp
Journal or Publication Title: Journal of global antimicrobial resistance
Journal Index: Pubmed
Identification Number: https://doi.org/10.1016/j.jgar.2020.03.011
ISSN: 2213-7173 (Electronic) 2213-7165 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.zaums.ac.ir/id/eprint/4094

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