Repository of Research and Investigative Information

Repository of Research and Investigative Information

Zahedan University of Medical Sciences

High prevalence of blaCMY AmpC beta-lactamase in ESBL co-producing Escherichia coli and Klebsiella spp. clinical isolates in the northeast of Iran

(2020) High prevalence of blaCMY AmpC beta-lactamase in ESBL co-producing Escherichia coli and Klebsiella spp. clinical isolates in the northeast of Iran. Journal of global antimicrobial resistance. pp. 477-482. ISSN 2213-7173 (Electronic) 2213-7165 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/32247080

Abstract

OBJECTIVE: The production of beta-lactamase enzymes such as AmpC beta-lactamases and extended-spectrum beta-lactamases (ESBLs) is among the main mechanisms for resistance to expanded-spectrum cephalosporins. The present study was conducted to investigate the prevalence and molecular epidemiology of plasmid-mediated AmpC beta (beta)-lactamase in ESBL co-producing Escherichia coli (E. coli) and Klebsiella spp. (Klebsiella pneumoniae and Klebsiella oxytoca) clinical isolates in the northeast of Iran. METHODS: A total of 602 E. coli and Klebsiella spp. clinical isolates were collected from three hospitals in Mashhad (northeast of Iran). A combination disk test (CDT) was performed for the phenotypic detection of ESBLs. Screening for the detection of AmpC beta-lactamases was performed by a susceptibility test to a cefoxitin disc among ESBL producing isolates. A confirmatory test for AmpC beta-lactamases was performed using the Mast(R) D68C test. Identification of plasmid-mediated AmpC cluster genes was done by multiplex polymerase chain reaction (PCR). RESULTS: Among 336 ESBL-producing strains, 230 (68.4) isolates were resistant to cefoxitin. Results of the Mast(R) D68C test showed that 30 (69/230) of cefoxitin-resistant isolates simultaneously exhibited ESBL and AmpC activity and 22 (51/230) of isolates probably showed multi-drug resistant (MDR) phenotype. Results of multiplex PCR among ESBL-positive isolates showed that, 16.7 (56/336) of isolates were positive for plasmid-borneampC cluster genes, and CMY (38) was the most frequent genotype of plasmid mediated AmpC. CONCLUSION: Findings of the study revealed that an increase in the prevalence of ESBL and AmpC co-producer in E. coli and Klebsiella spp. strains may become an important public health issue. Therefore, there is a vital need for surveillance of spread of these clinical isolates.

Item Type: Article
Keywords: AmpC beta-lactamases Escherichia coli Klebsiella spp extended spectrum beta-lactamases (ESBLs)
Page Range: pp. 477-482
Journal or Publication Title: Journal of global antimicrobial resistance
Journal Index: Pubmed
Volume: 22
Identification Number: https://doi.org/10.1016/j.jgar.2020.03.011
ISSN: 2213-7173 (Electronic) 2213-7165 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.zaums.ac.ir/id/eprint/4878

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